Furthermore, both the mitochondrial ROS scavenger MnTMPyP and the cytosolic ROS scavenger N-acetylcysteine (NAC) effectively reversed the hypoxia-induced increase in HIF1α and EMT features, as well as the decrease in CypD expression, thereby significantly reducing the invasive capacity of cancer cells (Fig. 3h, i). This evidence concerns the gene HIF1A and cancer.