To ensure that deterred tumor development was linked to pyroptosis led by the reduction of HOXC8, we performed immunohistochemistry to analyze the abundance of HOXC8, caspase-1, Ki67 and cleaved caspase-3 in tumors excised from Chol-NC and Chol-siHOXC8 groups. Here, HOXC8 is linked to neoplasm.