PMAIP1 and liver cancer: Furthermore, depletion of WSB2 through short hairpin RNA (shRNA)-mediated knockdown (KD) or CRISPR/Cas9-mediated knockout (KO) in prostate cancer C4-2B cells or liver cancer Huh-7 cells led to a marked increase in the steady-state levels of endogenous NOXA, without affecting other BCL-2 family proteins examined (Figure 2A–C, Figure 2—figure supplement 2A, B).