The analysis identified 156 pathways, suggesting that Juglone primarily influences CRC development through the regulation of the actin cytoskeleton, Rap1 signaling pathway, axon guidance, protein processing in the endoplasmic reticulum, cGMP-PKG signaling pathway, JAK-STAT signaling pathway, mTOR signaling pathway, phospholipase D signaling pathway, stem cell pluripotency regulation, NK cell-mediated cytotoxicity, and Th17 cell differentiation. The gene discussed is MTOR; the disease is colorectal carcinoma.