TNF and colon carcinoma: The aim of the study is to investigate the in vitro effects of this compound on HCT116 colon carcinoma cells, specifically, assessing its influence on cell viability (via MTT assay), migration (scratch assay), oxidative stress (TAS and TOS assays), inflammatory response (TNF-α gene expression via qRT-PCR), and tumor marker levels (CA19-9, CEA, CA72-4, and CYFRA 21-1 using CLIA).