RT-qPCR experiments showed that the mRNA expression levels of p53 downstream target genes, including apoptosis-related genes (PUMA and DR5), cell cycle-related genes (GADD45A and p21), and senescence-related genes (DEC1 and PAI1), were upregulated in ACY-1215-treated HCC cells (Figure 2C,D). Here, TP53 is linked to hepatocellular carcinoma.