After observing the expression of Nectin-2 and Nectin-4 in different tumor sites (membrane, cytoplasm, tumor edges) and regarding the heterogeneity of tissues after IHC staining, our next aim was to statistically analyze whether the difference in cytoplasmic and membrane expression between Nectin-2 and Nectin-4 is significant or not. The gene discussed is NECTIN4; the disease is neoplasm.