In this study, we identified that NTG-induced migraine is associated with the PI3K–Akt signaling pathway, cytokines and their receptor interactions, and nine key hub genes (Alb, Tgfb1, Cd4, Ptprc, Itgb1, Icam1, Col1a1, Pxdn, and Itgad) through transcriptomics. The gene discussed is ICAM1; the disease is migraine disorder.