Zhang et al.108designed a multifunctional nano-sonosensitizer protoporphyrin IX (PpIX) loaded with the calcium supplement CaO2 and DAC (lipo-(PpIX/CaO2/DAC)) to introduce Ca2+ into the cytoplasm to effect mitochondrial dysfunction and induce GSDME-dependent pyroptosis tumor therapy through calcium overload (Fig. 3B). Here, GSDME is linked to neoplasm.