Treatment with 25 μM or 50 μM DHA for 24 h induced dose-dependent SOX5 downregulation in NSCLC lines, most pronounced in A549 cells at 50 μM (p < 0.001; Figure 4B), while SOX5 expression remained unaltered in BEAS-2B cells (p > 0.99; Figure 4B) - a finding corroborated by Western blotting showing significant SOX5 suppression post-50 μM DHA treatment in all NSCLC lines (p < 0.0001; Figures 4C,D). Here, SOX5 is linked to non-small cell lung carcinoma.