In the present study, we analyze for the first time the impact of MTNR1B gene silencing in human myoblasts on melatonin-induced thermogenesis, which is previously demonstrated in the muscle from obese-diabetic rats, an in vivo model for the study of diabesity closely resembling human T2DM (Salagre et al., 2024b). The gene discussed is MTNR1B; the disease is type 2 diabetes mellitus.