To the best of our knowledge, we are the first to examine the effect of lncRNATUG1 on the transcriptome of HCC cells and have identified glycolysis as the most significantly affected pathway uponTUG1 depletion.TUG1-knockdown HCC cells exhibited a significant downregulation of several genes encoding glycolytic enzymes. Here, HK1 is linked to hepatocellular carcinoma.