To determine the optimal normalization strategy for western blot analysis in mature human adipocytes, housekeeping proteins previously validated for adipocyte and obesity research, such as parkinsonism associated deglycase (PARK7), enolase 1 (ENOA), and fumarylacetoacetase (FAH) were investigated [10]. The gene discussed is PARK7; the disease is obesity due to melanocortin 4 receptor deficiency.