To determine the optimal normalization strategy for western blot analysis in mature human adipocytes, housekeeping proteins previously validated for adipocyte and obesity research, such as parkinsonism associated deglycase (PARK7), enolase 1 (ENOA), and fumarylacetoacetase (FAH) were investigated [10]. This evidence concerns the gene FAH and obesity due to melanocortin 4 receptor deficiency.