We conducted differential gene expression to further characterize the NEUROD1-high (abbreviated ‘NE-variant’) and ASCL1-high (abbreviated ‘NE’) clusters observed with RPM cells (from cell lines and tumors) and found that the NE-variant cluster was associated with increased expression of genes highly expressed in variant SCLC (NEUROD1, SCG3, IGFBPL1, SSTR2, and MYC) (Figure 4—figure supplement 1D, Supplementary file 1). This evidence concerns the gene SCG3 and small cell lung carcinoma.