In immunolocalization studies variants in CUT2 domain of SATB2 localized to cytoplasm instead of nucleus suggesting that the nuclear localization signal of SATB2 resides in the CUT2 domain and that Msx1 promoter due to SATB2 variants may contribute to cleft palate and tooth agenesis in SATB2-associated syndrome (46) (Figure 1). This evidence concerns the gene SATB2 and cleft palate.