Consistent with these findings, the colony formation assay showed a notable increase in colony numbers in the OE-PRDX1 group (92 ± 7 colonies), while the sh-PRDX1 group exhibited significantly fewer colonies (36 ± 5 colonies) (Figure 6C), supporting PRDX1’s role in promoting HCC cell proliferation. The gene discussed is PRDX1; the disease is hepatocellular carcinoma.