It is noteworthy that the PI3K-AKT signaling pathway exhibited significant alterations across all examined organs (including the heart, ILN, kidneys, liver, lungs, MLN, muscles, spleen, SLN, and tonsils), which is similar to our previous findings in the Bama minipigs infection model [30], indicating that ASFV may regulate the PI3K-AKT signaling transduction pathway to evade the host's immune defense mechanisms. This evidence concerns the gene AKT1 and infection.