Based on the maximum selected rank statistic, the optimal cutoff values ​​for the most significant association of ALT, AST, the ALT/AST ratio, and TyG index with diabetes were determined to be 30.3 U/L, 27.9 U/L, 1.02, and 8.78, respectively (Supplementary Fig. 5–8). This evidence concerns the gene GPT and diabetes mellitus.