Using single-cell multiome (single-cell RNA-seq (scRNA-seq) and scATAC–seq), we analyzed 2,110 D0, 1,739 D14 and 2,344 D21 LNCaP+FOXA2 cells, whose transcriptome, respectively, aligned with clinical primary PCa, CRPC and NEPC, as expected46 (Extended Data Fig. 3d,e). This evidence concerns the gene FOXA2 and posterior cortical atrophy.