We infected PCa cell lines with FOXA2 OE lentivirus and observed a gradual transformation of AR-positive LNCaP and 22Rv1 cells to NEPC phenotype over the course of 28 days and of the AR-negative Du145 cells in only 7 days (Extended Data Fig. 2a,b and Supplementary Note 1). This evidence concerns the gene FOXA2 and posterior cortical atrophy.