VIM and cancer: To investigate the significance of C328 in EMT and cancer, we made a mutant encoding a C328S substitution in vimentin (C328S-VIM) and transduced in MCF-7 cells, which are devoid of endogenous vimentin (Usman et al., 2022a; Liu et al., 2015; Sivagurunathan et al., 2022), and are widely used as a model to study EMT (Guttilla et al., 2012; Kondaveeti et al., 2015).