Multiple breast cancer stem cell markers such as POU5F1 (Vezzoni and Parmiani, 2008), CD56 (Moghbeli et al., 2014), CD49f (Zhang et al., 2020), and CD201/PROCR (Hwang-Verslues et al., 2009) were also upregulated by C328-VIM, demonstrating increased stemness characteristics in MCF-7 cells as judged by the RNA-Seq analysis. The gene discussed is POU5F1; the disease is breast cancer.