Herein, we present a case of brain metastatic pulmonary SqCC with high programmed cell death ligand 1 (PD-L1, tumor proportion score [TPS]=75%, combined positive score [CPS]=75) expression and high tumor mutational burden (TMB-H, 28.49 Mut/Mb) who underwent sequential resection of brain and lung lesions after immunotherapy combined with chemotherapy. Here, CD274 is linked to neoplasm.