A key limitation of bulk RNA sequencing is its inability to resolve cellular heterogeneity, which precludes the identification of cell-specific changes in gene expression.71 The responses of heterogenous liver cell populations to WD-feeding, particularly in respect to hepatocyte zonality and the regulatory roles of parenchymal cells, remain critical areas of interest in understanding the link between AGT and liver steatosis. This evidence concerns the gene AGT and Hepatic steatosis.