This observation is consistent with several recent reports which indicate that early liver proliferation is a key driver of hepatic steatosis.56,64,65 Expression of Top2a, Cdc6, and Mki67 is dynamically regulated within the cell cycle,57,66,67 suggesting that the upregulation of these genes by WD feeding may occur subsequent to proliferative intracellular signaling. This evidence concerns the gene TOP2A and fatty liver disease.