Understanding how MG respond to and influence mechanical cues, and the extent to which TGF-β1 contributes to this feedback loop, is crucial for identifying therapeutic strategies in diseases such as glaucoma and proliferative vitreoretinopathy.Primary MG culture is an essential model to study the physiology and pathophysiology of these cells under controlled conditions. The gene discussed is TGFB1; the disease is myasthenia gravis.