To interrogate the mechanistic hierarchy between ITGB1-mediated mechano-signaling and G6PD-driven sorafenib resistance in HCC, stable ITGB1-knockdown models were generated in G6PD-overexpressing (oeG6PD) HCC-LM3 and HepG2 cell lines. Here, ITGB1 is linked to hepatocellular carcinoma.