TP53 and neoplasm: As 2 rounds of immunization with Ad-p53 DCs was shown to efficiently induce anti-p53 immune responses in mice19, Ad-p53 DCs were subcutaneously injected into the flanks of mice weekly for 4 weeks on days −21, −14, −7, and +6, before and after tumor inoculation (day 0) (Fig. 2D).