We next tested the prediction from the Chromatin Model that PARP7 ADP ribosylation of AR occurs upon DNA binding, using an AR exon2 loss-of-function mutation (V582F) from a French family with complete androgen insensitivity syndrome that maps to the DNA recognition helix (Fig. 5A; described originally as V581F) (Lumbroso et al, 1993). The gene discussed is TIPARP; the disease is androgen insensitivity syndrome.