As in yeast two‐hybrid experiments (Figure 6b) and in FRET‐FLIM experiments (Figure 6c), RACB‐CA‐5Q interacted with RIC171 in a similar manner as the parental RACB‐CA; we conclude that lipid binding through the PBR is required for PM association of RACB‐CA and for its capability to co‐recruit the ROP scaffold RIC171 to the cell periphery upon infection. Here, OPN1LW is linked to infection.