Through an analytical pipeline involving unsupervised clustering (Phenograph), spatial interaction analysis (Scimap), CN detection and SpatialScore metrics, the study revealed that regulatory T cells (Tregs) were enriched in stromal and peripheral tumour‐margin regions of non‐responding patients, with increased proximity to monocytes (p = .009) and CD8+ T cells (p = .009), while macrophages were more frequently associated with HLADR+ tumour cells (p = .01) in responders. This evidence concerns the gene CD8A and neoplasm.