To further verify the regulatory effect of NKD1 on downstream target genes of MYC, SW620 colon cancer cells were transfected with NC siRNA, NKD1 siRNA-1, or NKD1 siRNA-2, and several MYC downstream target genes, such as PTMA [24], SLC1A5 [25], RIOX2 [26], MAX, BOP1 [27], and SOX2 [28] on the basis of previously reported methods and analyzed by qRT-PCR and western blotting. This evidence concerns the gene NKD1 and malignant colon neoplasm.