To investigate whether PPARδ regulates the activity of the NKD1 promoter, we transfected the pGL3-598 promoter plasmid, pcDNA3.1 plasmid, and pcDNA3.1-PPARδ plasmid into colon cancer HCT116 and SW480 cells, and the NKD1 promoter activity regulated by PPARδ was detected via dual-luciferase reporter gene analysis. This evidence concerns the gene NKD1 and colonic neoplasm.