ALKBH5 promotes the demethylation of WIF-1 mRNA, enhances its transcription, and inhibits the Wnt signaling pathway in AsPC-1 and PANC-1 cells, thereby increasing the sensitivity of pancreatic cancer cells to gemcitabine.549 Moreover, ALKBH5 affects chemotherapy resistance by regulating the stemness of cancer cells. The gene discussed is WIF1; the disease is cancer.