We also disrupted the same three genes in the renal cell carcinoma line SW156, which expresses moderate levels of ACE2 and LGMN, but low to almost undetectable levels of TMPRSS2 (Fig 3B) and serves as a model for SARS-CoV-2 infection of the kidney [42,43]. Here, LGMN is linked to hereditary clear cell renal cell carcinoma.