ACE2 and viral infectious disease: Using a monoclonal 293T cell line with ACE2 genetically disrupted (hereafter ACE2KO 293T), we found that overexpression of ACE2 could induce viral infection, but overexpression of either KCNA6 or LGMN in this context did not promote entry by SARS-CoV-2 live virus over background levels (Fig 5A).