ACE2 and infection: We then confirmed that these cells were capable of sgRNA-directed gene activation (S1D Fig) and ACE2-dependent infection using a SARS-CoV-2 D614G-mutant-Spike with a 19 amino acid C-terminal deletion (hereafter, D614G-Spike) pseudotyped lentivirus that confers zeocin resistance and GFP expression (S1C Fig).