The current study aimed to examine the effects of a 12‐week intake of pure TB in combination with a low‐calorie diet on serum levels of HDL‐c2, HDL‐c3, the HDL‐c2/HDL‐c3 ratio, and the gene expression of peroxisome proliferator‐activated receptor alpha (PPAR‐α) and silent information regulator 1 (Sirt1) in overweight and obese adults with MetS. This evidence concerns the gene PPARA and metabolic syndrome.