Although body weight, edema, blood pressure (BP), and cardiothoracic ratio (CTR) have been used as indicators of fluid volume, this study focused on BNP, which is secreted by cardiomyocytes in response to ventricular hypertrophy, inflammation, or distention of the left ventricle, and myocardial injury [12]. This evidence concerns the gene NPPB and Ventricular hypertrophy.