To gain insight into the gene amplification of the two main EBL proteins currently known to be involved in reticulocyte invasion (DBP and EBP2), this study assessed the copy number variation of the dbp and ebp2 genes in P. vivax isolates from different endemic areas of the Amazon region, where malaria transmission ranges from hypoendemic to mesoendemic [31]. The gene discussed is DBP; the disease is malaria.