In accordance with this, the levels of pro-inflammatory factors, such as CXCL1, IL-36 G (IL-36γ, TNF-α, IL-6, and IL-1β, were significantly increased in the CDI group and decreased in the CDI +Iron diet group (Figure 2d–h), suggesting that high iron effectively prevented the recruitment of neutrophils to the infection site, thus reducing the inflammatory response. Here, IL1B is linked to clostridium difficile infection.