Single-cell RNA sequencing (scRNA-seq) has emerged as a powerful tool for studying the heterogeneity of cell populations within tumors, providing high-resolution information on the gene expression profiles of individual cells.13,14 This method is particularly effective in identifying specific immune cell types associated with the presence of Fn in colorectal tumors, thereby illuminating the interactions between Fn and the systemic immune response. This evidence concerns the gene FN1 and colorectal neoplasm.