Coincident with the broad trends described above, CyCIF data revealed a high level of specimen-to-specimen differences in the proportions of cells positive for one or more tumor state markers MART1, NGFR, SOX9, PRAME and S100A1 or the KI67+ proliferation marker (Fig. 2C, Supplementary Fig. S2D,E). This evidence concerns the gene PRAME and neoplasm.