Therefore, we confirmed the expression of LGR7/RXFP1 in cancer cell lines using immunoblotting, including the human pancreatic cancer cell lines (SU86.86, Panc-1, AsPC-1, AsPC-1-CD44v6, and MIA PaCa-2 cells), bile duct cancer cell lines (TFK-1, HuCCT-1, and NOZ), imhPSCs, and LNCap (Figure 2C) (36). Here, RXFP1 is linked to bile duct cancer.