The survival time and the changes of tumor size, DC counts in tumor tissue, serum cytokine levels (IFN-γ and IL-4), and Th1/Th2 ratio were compared among Control group (CLM mice given sham treatment), Group A (CLM mice given mouse GM-CSF injection of 250 μg/day for three consecutive days), Group B (CLM mice given cryoablation treatment), and Group C (CLM mice given combined cryoablation and GM-CSF treatment). This evidence concerns the gene IL4 and neoplasm.