To further investigate the molecular mechanism by which LncDARS‐AS1 stabilizes ATP1A1 protein in osteosarcoma cells via the ubiquitin‐proteasome system (UPS), co‐immunoprecipitation (co‐IP) followed by mass spectrometry analysis was conducted to compare LncDARS‐AS1‐knockdown cells with control cells. This evidence concerns the gene ATP1A1 and osteosarcoma.