These results lead us to three main interpretations: dying neurons are required to enhance microglial activity (6‐OHDA alone is not enough); neuronal TRPM2 is involved in the mechanism of 6‐OHDA‐induced cell death; and microglial TRPM2 may be involved in microglial activation; thus, inhibiting the channel before PD induction favors a more homeostatic microglial state, which alleviates the 6‐OHDA effect observed in the co‐culture. Here, TRPM2 is linked to Parkinson disease.