Cytokine analysis of tumor tissues revealed that the combination of Liensinine and immunotherapy significantly elevated levels of pro-inflammatory cytokines, including TNF-α, IL-12, IFN-γ, and granzyme B, while reducing the levels of immunosuppressive cytokines, such as IL-10 and TGF-β (Fig. 7J). This evidence concerns the gene GZMB and neoplasm.