However, while previous publications suggest that CDK16 promotes tyrosine phosphorylation of PRC1 at the T481 site [16, 25], we constructed several PRC1 (T429A, S431A, and T495A) variants and identified that PRC1 phosphorylation at T429 was indispensable for PRC1 activation and its oncogenic role in NSCLC. The gene discussed is PRC1; the disease is non-small cell lung carcinoma.