To elucidate the correlation between TAMs infiltration and ERα expression in BLCA, we detected the expression of CD68/CD163 and ERα in 40 BLCA human samples, including 24 muscle-invasive bladder cancer (MIBC) samples and 16 non-muscle-invasive bladder cancer (NMIBC) samples, and the results showed that the expression of ERα was positively correlated with TAMs infiltration (Fig. 1I-J). This evidence concerns the gene CD68 and bladder transitional cell carcinoma.