In AD, several established biomarkers exhibited significant associations, including 14-3-3 protein YWHAH (β = 0.10, P = 5.9 × 10−26), SMOC1 (β = 0.20, P = 1.6 × 10−21) and PPP3R1 (β = 0.10, P = 4.3 × 10−6)10,19. This evidence concerns the gene PPP3R1 and Alzheimer disease.