EGF and non-small cell lung carcinoma: As expected, compared with control cells, EGF-treated and TGF-β-treated NSCLC cells lost their cobblestone morphology and acquired a spindle-shaped pattern with motivated F-actin rearrangement, a lower level of the epithelial marker ECAD and a higher level of the mesenchymal marker Vimentin (Fig. 2b and Supplementary Fig. 2a, b).