Furthermore, Western blot analysis of apoptosis-related proteins, including cleaved-PARP, cleaved-caspase 3, cleaved-caspase 7, and cleaved-caspase 9, revealed that the expression levels of these proteins were consistently higher in the combination therapy group, underscoring the enhanced apoptotic response elicited by the synergistic interaction of RP-6306 and gemcitabine in pancreatic cancer cells (Fig. 5D). This evidence concerns the gene CASP9 and familial pancreatic carcinoma.