Given cholesterol’s critical role in maintaining lipid-raft integrity and regulating various signaling cascades by controlling receptor localization and trafficking between raft and non-raft regions [24], we hypothesized that reduced cholesterol levels could alter TGF-β receptor distribution across membrane compartments to modulate TGF-β activity in HCT116-MD and HD cells. This evidence concerns the gene TGFB1 and Menkes disease.