To determine the involvement of NFAT5 in IEC functions, we downregulated its expression in the human colorectal adenocarcinoma cell lines HT-29 and Caco-2 using siRNAs (Supplemental Figure 1, A and B; supplemental material available online with this article; https://doi.org/10.1172/JCI183093DS1). This evidence concerns the gene NFAT5 and colorectal adenocarcinoma.