Herein, we extend these observations by comparing different tumor regions, where 3 classes of CD8+ T cell exclusion from the tumor nest can be observed during formation of the immune desert: type I restricted inflamed with NOS2+ tumor edge and high tumor COX2 expression, type II NOS2– tumor edge with high tumor COX2 expression, and type III with NOS2– tumor edge, low sporadic COX2 expression, and few proximal stromal lymphoid cells < 500 μm (<1%) (Figure 6, A and B). The gene discussed is NOS2; the disease is neoplasm.